Invitrogen FGF4 Human ELISA Kit, 96 Tests

Invitrogen FGF4 Human ELISA Kit, 96 Tests

The Human Fibroblast Growth Factor 4 (Hu FGF4) ELISA quantitates Hu FGF4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF4.

Principle of the method

The Human FGF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents    

• Human FGF-4 Antibody Coated wells, 96-well plate, 1 plate 
• Human FGF-4 Biotin Conjugate, 2 vials 
• Human FGF-4 Standard, recombinant human FGF-4, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 30 mL 
• Assay Diluent B (5X), 15 mL 
• Streptavidin-HRP (120X), 0.2 mL
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2