Invitrogen™ Fluo-3, AM, cell permeant
On demand
Order number :
F14218
Invitrogen™ Fluo-3, AM, cell permeant
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+
signaling, in flow cytometry experiments involving photoactivation of
caged chelators, second messengers, and neurotransmitters, and for
cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with
the two chlorine substituents replaced by fluorines, which results in
increased fluorescence excitation at 488 nm and consequently higher
fluorescence signal levels. Cells may be loaded with the AM ester forms
of these calcium indicators by adding the dissolved indicator directly
to dishes containing cultured cells. These indicators are useful for
fluorescence and confocal microscopy, flow cytometry, and microplate
screening applications.
Features
- For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
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