Invitrogen™ Fluo-5F, Pentapotassium Salt, cell impermeant
On demand
Order number :
F14221
Invitrogen™ Fluo-5F, Pentapotassium Salt, cell impermeant
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-5F, fluo-5N, and fluo-4ff are analogs of fluo-4 with lower Ca2+-binding affinity, making them suitable for detecting intracellular calcium levels in the 1 µM to 1 mM range that would saturate the response of fluo-3 and fluo-4. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection or our Influx™ pinocytotic cell-loading reagent. These indicators are compatible with excitation at 488 nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications.
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
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