Invitrogen™ FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins
On demand
Order number :
F30751
Invitrogen™ FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins
The FluoReporter® Biotin Quantitation Assay Kit provides a sensitive fluorometric assay for determining the number of biotin labels on nucleic acid samples (i.e., cDNA). The assay uses Biotective™ Green reagent, which contains a fluorescent dye that is quenched by ligands occupying the biotin binding sites. In the presence of biotin, the quencher dye ligands are displaced and fluorescence occurs. The fluorescence is proportional to the amount of added biotin. The results can be read with any fluorescence-based microplate reader capable of detecting FITC dye.
Features
- Can detect from 4 to 80 picomoles of biotin in a sample (50-fold higher sensitivity than HABA biotin-binding assay)
- Can be applied to as little as 13 ng of biotin-labeled nucleic acid (more is needed for lower degrees of labeling)
- Excitation/emission maxima of the reagent is 495/519 nm
- Supplied with a biotinylated positive control for standard curve
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