Invitrogen™ FluoReporter™ lacZ Flow Cytometry Kit

Invitrogen™ FluoReporter™ lacZ Flow Cytometry Kit

The FluoReporter® lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in single cells using flow cytometry. This kit is accompanied by a license to practice patented techniques for loading FDG by hypotonic shock and improving retention of fluorescein in lacZ-positive cells.

Contents

• Contains 1 vial FDG (0.5 mL, 20 mM in water/DMSO/ethanol, 8:1:1, v/v), 1 vial PETG (2.0 mL, 50 mM in water), chloroquine diphosphates (1.0 mL, 30 mM in water) and 1 vial of propidium iodide (1.0 mL, 150 µM in water).