Invitrogen™ FluoSpheres™ Polystyrene Microspheres, 15 µm, Yellow Fluorescent (515/534), For Blood Flow Determination
On demandInvitrogen™ FluoSpheres™ Polystyrene Microspheres, 15 µm, Yellow Fluorescent (515/534), For Blood Flow Determination
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.
FluoSpheres™ microspheres in the 10–15 µm size range were designed specifically for regional blood flow studies in tissues and organs. Typically for these types of studies, the microspheres are injected at desired locations in the circulatory system and eventually lodge in the capillaries, where they can be extracted and the fluorescence quantified. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres. Multiple (up to seven or more with good spectral correction) simultaneously circulating FluoSpheres™ microspheres can be discriminated. Microspheres that have been perfused or injected into tissues and organs retain their fluorescence following histological serial sectioning. Our FluoSpheres™ microspheres for blood flow determination, which are available in eleven distinguishable fluorescent colors, are also compatible with blood flow analyzer systems that perform automated extraction and analysis.
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