Invitrogen™ FluoZin™-3, AM, cell permeant, 100 mL

Invitrogen™ FluoZin™-3, AM, cell permeant, 100 mL

alamarBlue HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. High background fluorescence caused by various contaminants reduces the performance of resazurin-based reagents. To improve the performance, an innovative purification process was developed that removes these contaminants. This highly purified, non-toxic resazurin was used in the standard alamarBlue formulation, creating the multiple-component alamarBlue HS Cell Viability Reagent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. The alamarBlue HS Cell Viability Reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi

Features

• Removal of contaminants from resazurin—displays a >50% reduction in background fluorescence
• Signal-to-background ratio increased by >100%—results in large assay signal window
• Highly sensitive reagent with a linear response—detects as few as 20 cells per well
• Convenient add-and-read format—no mixing, no washing, no cell lysis, and compatible with either fluorescence- or absorbance-based instrumentation
• Measures viability from many diverse cell types—including mammalian cells, bacteria, plant, and fungi
• For Use With (Equipment): Microplate Reader, Spectrophotometer, HTS Reader