Invitrogen Fractalkine-Cell Lysates (CX3CL1) Rat ELISA Kit, 10 x 96 Tests

Invitrogen Fractalkine-Cell Lysates (CX3CL1) Rat ELISA Kit, 10 x 96 Tests

The Rat Fractalkine-Cell Lysate (CX3CL1) ELISA quantitates Rt Fractalkine in rat cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Rt Fractalkine.

Principle of the method

The Rat Fractalkine solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10%    
• Shipping condition: Wet or Dry Ice    
• Storage: 2-8°C

Contents    

• Rat Fractalkine Antibody Coated wells, 96-well plate, 1 plate
• Rat Fractalkine Biotin Conjugate, 2 vials
• Rat Fractalkine Standard, recombinant rat fractalkine, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Sample Diluent Buffer (5X), 10 mL
• Assay Diluent (5X), 15 mL
• Cell lysate buffer (2X), 5 mL
• Streptavidin-HRP (240X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2