Invitrogen Galectin 3 Mouse ELISA Kit, 5 x 96 Tests
On demandInvitrogen Galectin 3 Mouse ELISA Kit, 5 x 96 Tests
The Mouse Galectin-3 (LGALS3) ELISA quantitates Ms Galectin-3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Galectin-3.
Principle of the method
The
Mouse Galectin-3 solid-phase sandwich ELISA (enzyme-linked
immunosorbent assay) is designed to measure the amount of the target
bound between a matched antibody pair. A target-specific antibody has
been pre-coated in the wells of the supplied microplate. Samples,
standards, or controls are then added into these wells and bind to the
immobilized (capture) antibody. The sandwich is formed by the addition
of the second (detector) antibody, a substrate solution is added that
reacts with the enzyme-antibody-target complex to produce measurable
signal. The intensity of this signal is directly proportional to the
concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV:<10%
- Homogenous (no wash): No
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Mouse Galectin-3 Antibody Coated wells, 96-well plate, 1 plate
- Mouse Galectin-3 Biotin Conjugate, 2 vials
- Mouse Galectin-3 Standard, recombinant mouse galectin-3, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (400X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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