Invitrogen GDF15 Human ELISA Kit, 5 x 96 Tests, 100 µL
On demandInvitrogen GDF15 Human ELISA Kit, 5 x 96 Tests, Sample Volume 100 µL
The Human GDF15 ELISA quantitates Hu GDF15 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GDF15.
Principle of the method
The Human GDF15 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human GDF-15 Antibody Coated wells, 96-well plate, 1 plate
- Human GDF-15 Biotin Conjugate, 2 vials
- Human GDF-15 Standard, recombinant human GDF-15, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent (5X), 15 mL
- Streptavidin-HRP (500X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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