Invitrogen™ GLOBINclear™ Kit, mouse/rat, for globin mRNA depletion

Invitrogen™ GLOBINclear™ Kit, mouse/rat, for globin mRNA depletion

The Ambion® GLOBINclear™-Mouse/Rat Kit employs a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood. The kit includes sufficient reagents for 20 reactions.

It is widely known that expression array data generated from whole blood total RNA samples have reduced detection sensitivity compared to data from fractionated blood samples. Examination of whole blood total RNA reveals a dominant band in the 600–700 base region. This band represents globin mRNA, which is expressed at high levels in red blood cells (RBCs) and reticulocytes (RBC precursors). Up to 70% of the mRNA (by mass) in whole blood total RNA are globin transcripts. 

Features

• Remove >95% of unwanted globin mRNA from whole blood total RNA
• Detect up to 50% more genes—analyze previously undetected genes
• Achieve greater representation of the genome, low 3'/5' ratios
• Avoid RNase H treatments that can degrade mRNA and alter expression profiles

Contents

• Capture Oligo Mix (-20°C): 20 µl 
• Streptavidin Magnetic Beads (4°C—do not freeze): 600 µl 
• 2X Hybridization Buffer (room temperature): 1.5 ml 
• Streptavidin Bead Buffer (room temperature): 1.5 ml 
• Nuclease-free Water (-20°C, 4°C, or room temperature): 1.75  mL
• RNA Binding Beads (4°C—do not freeze): 200 µl 
• RNA Wash Solution Concentrate (4°C or room temperature): 5 ml 
• Elution Buffer (4°C or room temperature): 1 ml 
• RNA Bead Buffer (room temperature): 80 µl 
• RNA Binding Buffer Concentrate (room temperature): 4 ml 
• 1.5-ml Non-stick Tubes (room temperature): 100 Tubes