Invitrogen GLP-1 Multispecies ELISA Kit, 96 Tests
On demandInvitrogen GLP-1 Multispecies ELISA Kit, 96 Tests
The Human Glucagon Like Peptide-1 (Hu GLP-1) ELISA quantitates Hu GLP-1
in human serum, plasma, buffered solution, or cell culture medium. The
assay will exclusively recognize both natural and recombinant Hu GLP-1.
Principle of the method
The
Human GLP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent
assay) is designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody has been pre-coated in
the wells of the supplied microplate. Samples, standards, or controls
are then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 3.8%
- Intraassay CV: 3.8%
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Sample Diluent
- Assay Buffer concentrate
- Biotinylated Detection Antibody
- SAV-HRP
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers