Invitrogen™ Glutathione Fluorescent Detection Kit

Invitrogen™ Glutathione Fluorescent Detection Kit

The Glutathione Detection research-use-only kit is a fluorescent assay designed for the quantification and detection of glutathione in whole blood, serum, plasma, erythrocytes, urine, cell lysates and tissue samples.

This complete, ready-to-use kit includes black 96-well plate(s), glutathione standard, detection reagent, assay buffer, and other components to perform the assay. A 96-well microplate reader capable of reading fluorescent emission at 510 nm, with excitation at 390 nm, is required for use of this kit.

Features

• Sample types: whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples
• Product Type: Glutathione Fluorescent Detection Kit
• Gene ID (Entrez): Non-Gene
• Gene Symbol: Non-Gene
• Sensitivity: 45 nM
• Standard curve range: 0.2 uM–25 uM
• Reactivity: species independent
• Storage: Store at –20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Target Kit Named: Glutathione
• For Use With (Equipment): Microplate Reader
• Immunoassay Kit Format: Detection Kit

Content

• Glutathione Standard; 250 μM glutathione in a special stabilizing solution, 100 μL
• Black 96-well Half Area Plate, 1 plate
• Detection Reagent; r econstitute with Dry DMSO, 2 vials
• Dry DMSO (Dimethyl sulfoxide), 4 mL
• Assay Buffer Concentrate (2X), 35 mL
• NADPH Concentrate (10X): reduced β-nicotinamide adenine dinucleotide 2’-phosphate, 300 μL
• Glutathione Reductase Concentrate (10X), 300 μL
• Oxidized Glutathione Control, 300 μL