Invitrogen Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555, 1 mg
On demandInvitrogen Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555, 1 mg
To minimize cross-reactivity, the goat anti-rabbit IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
MoreThere are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/16a455c8-b0f0-4d24-a93b-6654ca66f214.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red-X, 1 mg
$ 263
On demand
![](https://laboshop.ae/public/storage/files/7615b8cc-17be-4cfc-ba09-de44d9097288.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555, 1 mg
$ 431
On demand
![](https://laboshop.ae/public/storage/files/5570ecd9-ca3a-4b83-91a7-9c51f632ef37.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red, 1 mg
$ 260
On demand
![](https://laboshop.ae/public/storage/files/62b0a87a-3982-441c-9494-f83f8fbf5005.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP, 50 μg, 1 mg/mL
$ 108
On demand
![](https://laboshop.ae/public/storage/files/a8a69a18-c0ee-4213-905d-0ab72c55a689.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 405, 1 mg
$ 424
On demand
![](https://laboshop.ae/public/storage/files/4edb80a1-38db-4034-b0b4-9d77e7c7dad7.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647, 1 mg
$ 343
On demand
![](https://laboshop.ae/public/storage/files/acdcdca8-ddb9-4341-b53e-7be2076fa7a3.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC, 1 mg
$ 248
On demand
![](https://laboshop.ae/public/storage/files/e6564e07-7877-4814-bdbb-d481e55357db.jpg)
Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3, 1 mg
$ 262