Invitrogen Granzyme A Human ELISA Kit, 96 Tests
On demandInvitrogen Granzyme A Human ELISA Kit, 96 Tests
The Human Granzyme A ELISA quantitates Hu Granzyme A in human serum,
plasma, or cell culture medium. The assay will exclusively recognize
both natural and recombinant Hu Granzyme A.
Principle of the method
The
Human Granzyme A solid-phase sandwich ELISA (enzyme-linked
immunosorbent assay) is designed to measure the amount of the target
bound between a matched antibody pair. A target-specific antibody has
been pre-coated in the wells of the supplied microplate. Samples,
standards, or controls are then added into these wells and bind to the
immobilized (capture) antibody. The sandwich is formed by the addition
of the second (detector) antibody, binding to the target on a different
epitope from the capture antibody. A conjugated enzyme has been
incorporated into the assay. After incubation and washing steps to rid
the microplate of unbound substances, a substrate solution is added that
reacts with the enzyme-antibody-target complex to produce measurable
signal. The intensity of this signal is directly proportional to the
concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 4.9%
- Intraassay CV: 8.5%
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard (2 vials)
- Standard Dilution Buffer
- Biotinylated Detection Antibody
- SAV-HRP
- HRP Diluent
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers
There are no specifications
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