Invitrogen Growth Hormone Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen Growth Hormone Human ELISA Kit, 10 x 96 Tests
The Human Growth Hormone (Hu GH) ELISA quantitates Hu GH in human serum,
plasma, or cell culture medium. The assay will exclusively recognize
both natural and recombinant Hu GH.
Principle of the method
The
Human GH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay)
is designed to measure the amount of the target bound between a matched
antibody pair. A target-specific antibody has been pre-coated in the
wells of the supplied microplate. Samples, standards, or controls are
then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human Growth hormone Antibody Coated wells, 96-well plate,10 plate
- Human Growth hormone Biotin Conjugate, 20 vials
- Human Growth hormone Standard, recombinant human growth hormone, 20 vials
- Wash Buffer Concentrate (20X), 12 x 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 10 x 30 mL
- Assay Diluent B (5X),10 x 15 mL
- Streptavidin-HRP (200X), 10 x 0.2 mL
- TMB Substrate,10 x 12 mL
- Stop Solution, 10 x 8 mL
- Adhesive Plate Covers, 20
There are no specifications
There are no report
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