Invitrogen HB-EGF Human ELISA Kit, 10 x 96 Tests

Invitrogen HB-EGF Human ELISA Kit, 10 x 96 Tests

The Human HB-EGF ELISA quantitates Hu HB-EGF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HB-EGF.

Principle of the method

The Human HB-EGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents    

• Human HB-EGF Antibody Coated wells, 96-well plate, 10 plate 
• Human HB-EGF Biotin Conjugate, 20 vials
• Human HB-EGF Standard, recombinant human HB-EGF, 20 vials 
• Wash Buffer Concentrate (20X), 12 x 25 mL 
• Assay Diluent D (5X), 10 x 15 mL 
• Assay Diluent C, 10 x 30 mL 
• Assay Diluent B (5X), 10 x 15 mL
• Streptavidin-HRP (300X), 10 x 0.2 mL 
• TMB Substrate, 10 x 12 mL 
• Stop Solution, 10 x 8 mL 
• Adhesive Plate Covers, 20