Invitrogen HCC-1/CCL14 Human ELISA Kit, 96 Tests

Invitrogen HCC-1/CCL14 Human ELISA Kit, 96 Tests

The Human HCC-1/CCL14 ELISA quantitates Hu HCC-1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HCC-1.

Principle of the method

The Human HCC-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Human CCL14 Antibody Coated wells, 96-well plate, 1 plate 
• Human CCL14 Biotin Conjugate, 2 vials 
• Human CCL14 Standard, recombinant human CCL14, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL 
• Assay Diluent A (contains 0.09% sodium azide), 30 mL 
• Assay Diluent B (5X), 15 mL 
• Streptavidin-HRP (400X), 0.2 mL
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2