Invitrogen hCG alpha Human ELISA Kit, 96 Tests
On demandInvitrogen hCG alpha Human ELISA Kit, 96 Tests
The Human Chorionic Gonadotropin (hCG) ELISA quantitates Hu CG in human
serum, plasma, or cell culture medium. The assay will exclusively
recognize both natural and recombinant Hu CG.
Principle of the method
The
Human CG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay)
is designed to measure the amount of the target bound between a matched
antibody pair. A target-specific antibody has been pre-coated in the
wells of the supplied microplate. Samples, standards, or controls are
then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human hCG Antibody Coated wells, 96-well plate,1 plate
- Human hCG Biotin Conjugate, 2 vials
- Human hCG Standard, recombinant human hCG, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (700X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
There are no specifications
There are no report
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