Invitrogen HGH Human ELISA Kit, 96 Tests

Invitrogen HGH Human ELISA Kit, 96 Tests

The Human Growth Hormone (Hu GH) ELISA quantitates Hu GH in human serum and plasma. The assay will exclusively recognize both natural and recombinant Hu GH.
Principle of the method
The Human GH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 6.6%
• Intraassay CV: 5.2%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents    

• Pre-coated 96 well plate
• Standard
• Standard Dilution Buffer
• Incubation Buffer
• HRP conjugated Detection Antibody
• Wash Buffer
• Chromogen
• Stop Solution
• Controls