Invitrogen HGH Human ELISA Kit, 96 Tests
On demandInvitrogen HGH Human ELISA Kit, 96 Tests
The Human Growth Hormone (Hu GH) ELISA quantitates Hu GH in human serum
and plasma. The assay will exclusively recognize both natural and
recombinant Hu GH.
Principle of the method
The
Human GH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay)
is designed to measure the amount of the target bound between a matched
antibody pair. A target-specific antibody has been pre-coated in the
wells of the supplied microplate. Samples, standards, or controls are
then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 6.6%
- Intraassay CV: 5.2%
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Standard Dilution Buffer
- Incubation Buffer
- HRP conjugated Detection Antibody
- Wash Buffer
- Chromogen
- Stop Solution
- Controls