Invitrogen HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780, 25 Tests, eBioscience™
On demand
Order number :
47-9956-41
Invitrogen HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780, 25 Tests, eBioscience™
The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.
Features
- APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
- Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
- Recommended Isotype Control: Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor 780, eBioscience™.
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