Invitrogen Human Fc epsilon RI ELISA Kit, 10 x 96 Tests

Invitrogen Human Fc epsilon RI ELISA Kit, 10 x 96 Tests

The Human Fc epsilon RI ELISA quantitates Fc epsilon RI in human serum, plasma, and cell culture medium.
Principle of the method
The Human Fc epsilon RI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 6.8%
• Intraassay CV: 6.1%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents    

• Pre-coated 96 well plate
• Standard
• Detection antibody (chimeric IgE)
• IgE - HRP conjugate
• Assay Buffer concentrate
• Wash Buffer concentrate
• Substrate solution
• Stop Solution
• Adhesive Plate Covers