Invitrogen Human Granzyme B ELISA Kit, 96 Tests

Invitrogen Human Granzyme B ELISA Kit, 96 Tests

The Human Granzyme B ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human Granzyme B.

Principle of the method

The Human Granzyme B ELISA Kit (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: 6.8%
• Intraassay CV: 6.2%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Pre-coated 96 well plate
• Standard
• Detection antibody (monoclonal antibody to human Granzyme B)
• Biotin-Conjugate anti-human Granzyme B monoclonal antibody
• Streptavidin-HRP
• human Granzyme B Standard lyophilized
• Wash Buffer Concentrate 20x
• Dilution Buffer Concentrate 5x
• Substrate Solution
• Stop Solution
• Adhesive Films