Invitrogen Human Serpin A4 Human ELISA Kit, 5 x 96 Tests
On demandInvitrogen Serpin A4 Human ELISA Kit, 5 x 96 Tests
The Human Serpin A4 ELISA quantitates Hu Serpin A4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Serpin A4.
Principle of the method
The Human Serpin A4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV: <10%
- Homogenous (no wash): No
- Storage: 2-8°C
Contents
- Human Serpin A4 Antibody Coated wells, 96-well plate, 1 plate
- Human Serpin A4 Biotin Conjugate, 2 vials
- Human Serpin A4 Standard, recombinant human serpin A4, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (600X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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