Invitrogen ICAM-1 Mouse ELISA Kit, 10 x 96 Tests

Invitrogen ICAM-1 Mouse ELISA Kit, 10 x 96 Tests

The Mouse Intercellular Adhesion Molecule 1 (Ms ICAM-1) ELISA quantitates Ms ICAM-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ICAM-1.
Principle of the method
The Mouse ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Mouse ICAM-1 Antibody Coated wells, 96- well plate, 10 plate
• Mouse ICAM-1 Biotin Conjugate, 20 vials
• Mouse ICAM-1 Standard, recombinant mouse ICAM-1, 20 vials
• Wash Buffer Concentrate (20X), 12 x 25 mL
• Assay Diluent D (5X), 10 x 15 mL
• Assay Diluent B (5X), 10 x 15 mL
• Streptavidin-HRP (400X), 10 x 0.2 mL
• TMB Substrate, 10 x 12 mL
• Stop Solution, 10 x 8 mL
• Adhesive Plate Covers, 20