Invitrogen ICAM-1 Rat ELISA Kit, 96 Tests

Invitrogen ICAM-1 Rat ELISA Kit, 96 Tests

The Rat InterCellular Adhesion Molecule 1 (Rt ICAM-1) ELISA quantitates Rt ICAM-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt ICAM-1.
Principle of the method
The Rat ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Rat ICAM-1 Antibody Coated wells, 96-well plate, 1 plate
• Rat ICAM-1 Biotin Conjugate, 2 vials
• Rat ICAM-1 Standard, recombinant rat ICAM-1, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (400X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2