Invitrogen IFN beta Human ELISA Kit, 96 Tests

Invitrogen IFN beta Human ELISA Kit, 96 Tests

The Human Interferon beta (Hu IFNβ) ELISA quantitates Hu IFNβ in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFNβ.
Principle of the method
The Human IFNβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <8%
• Intraassay CV: <8%

Contents    

• Pre-coated 96 well Microplate
• Detection Antibody Concentrate
• Anti-IgG-HRP Concentrate
• Concentrate Diluent
• Standard
• Wash Buffer Concentrate
• Substrate Solution
• Sample Diluent
• Stop Solution
• Adhesive Plate Covers