Invitrogen IFN gamma Mouse ELISA Kit, 5 x 96 Tests

Invitrogen IFN gamma Mouse ELISA Kit, 5 x 96 Tests

The Mouse Interferon Gamma (Ms IFNγ) ELISA quantitates Ms IFNγ in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IFNγ.
Principle of the method
The Mouse IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: 6.5%
• Intraassay CV: 3.2%

Contents    

• Antibody Coated Wells. 96-well plate. 1 plate
• Mouse IFN-g Biotin Conjugate. Contains 0.1% sodium azide. 11 mL
• Mouse IFN-g Standard. Contains 0.1% sodium azide. 2 vials
• Wash Buffer Concentrate (25X). 100 mL
• Standard Diluent Buffer. Contains 0.1% sodium azide. 25 mL
• Streptavidin-HRP (100X). 0.125 mL
• Streptavidin-HRP Diluent. Contains 3.3 mM thymol. 25 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB). 25 mL
• Stop Solution. 25 mL
• Adhesive Plate Covers.