Invitrogen IgA Mouse ELISA Kit, 10 x 96 Tests

Invitrogen IgA Mouse ELISA Kit, 10 x 96 Tests

The Mouse Immunoglobulin A (Ms IgA) ELISA quantitates Ms IgA in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IgA.
Principle of the method
The Mouse IgA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Mouse IgA Antibody Coated wells, 96-well plate, 10 plate
• Mouse IgA Biotin Conjugate, 20 vials
• Mouse IgA Standard, recombinant mouse IgA, 20 vials
• Wash Buffer Concentrate (20X), 12 x 25 mL
• Assay Diluent (5X), 10 x 15 mL
• Streptavidin-HRP (1500X), 10 x 0.2 mL
• TMB Substrate, 10 x 12 mL
• Stop Solution, 10 x 8 mL
• Adhesive Plate Covers, 20