Invitrogen IGFBP3 Human ELISA Kit, 96 Tests
On demandInvitrogen IGFBP3 Human ELISA Kit, 96 Tests
The Human Insulin-like Growth Factor Binding Protein 3 (Hu IGFBP3) ELISA
quantitates Hu IGFBP3 in human serum, plasma, or cell culture medium.
The assay will exclusively recognize both natural and recombinant Hu
IGFBP3.
Principle of the method
The
Human IGFBP3 solid-phase sandwich ELISA (enzyme-linked immunosorbent
assay) is designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody has been pre-coated in
the wells of the supplied microplate. Samples, standards, or controls
are then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human IGFBP-3 Antibody Coated wells, 96-well plate,1 plate
- Human IGFBP-3 Biotin Conjugate, 2 vials
- Human IGFBP-3 Standard, recombinant human IGFBP-3, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X),15 mL
- Streptavidin-HRP (320X), 0.2 mL
- TMB Substrate,12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2