Invitrogen IGFBP5 Rat ELISA Kit, 10 x 96 Tests

Invitrogen IGFBP5 Rat ELISA Kit, 10 x 96 Tests

The Rat Insulin-Like Growth Factor Binding Protein 5 (Rt IGFBP5) ELISA quantitates Rt IGFBP5 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IGFBP5.
Principle of the method
The Rat IGFBP5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Rat IGFBP-5 Antibody Coated wells, 96-well plate, 1 plate
• Rat IGFBP-5 Biotin Conjugate, 2 vials
• Rat IGFBP-5 Standard, recombinant rat IGFBP-5, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent (5X), 15 mL
• Streptavidin-HRP (300X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2