Invitrogen IGFBP6 Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen IGFBP6 Human ELISA Kit, 10 x 96 Tests
The Human Insulin-like Growth Factor Binding Protein 6 (Hu IGFBP6) ELISA quantitates Hu IGFBP6 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGFBP6.
Principle of the method
The Human IGFBP6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
Contents
- Human IGFBP-6 Antibody Coated wells, 96-well plate, 10 plate
- Human IGFBP-6 Biotin Conjugate, 20 vials
- Human IGFBP-6 Standard, recombinant human IGFBP- 6, 20 vials
- Wash Buffer Concentrate (20X), 12 x 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 10 x 30 mL
- Assay Diluent B (5X), 10 x 15 mL
- Streptavidin-HRP (500X), 10 x 0.2 mL
- TMB Substrate, 10 x 12 mL
- Stop Solution, 10 x 8 mL
- Adhesive Plate Covers, 20
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