Invitrogen IgG Subclass Human ELISA Kit, 2 x 96 Tests

Invitrogen IgG Subclass Human ELISA Kit, 2 x 96 Tests

The Human IgG Subclass Profile (Hu IgG) ELISA quantitates Hu IgG subclasses in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu igG1, IgG2, IgG3 or IgG4.
Principle of the method
The Human IgG ELISAs are designed to measure the amount of immunoglobulin in the sample. Each Immunoglobulin subunit, IgG1, IgG2, IgG3 and IgG4 are added to individual wells and adsorbed to the bottom of the microplate well. Samples, standards, or controls are then added into these wells and bind to the immobilized IgG subunit. Following a wash step, anti-human IgG antibodies conjugated to HRP is added. Following another wash step, a TMB substrate solution is added that reacts with the complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents    

• Pre-coated 96 well plates
• Standard
• Sample Diluent
• Assay Buffer concentrate
• Serum Control
• MAbs to IgG Subclasses(4)
• Peroxidase-conjugated Antibody
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers