Invitrogen IL-1 beta Human ELISA Kit, High Sensitivity, 96 Tests
On demandInvitrogen IL-1 beta Human ELISA Kit, High Sensitivity, 96 Tests
The Human High Sensitivity Interleukin 1 beta (Hu HS IL-1β) ELISA quantitates Hu HS IL-1β in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-1β.
Principle of the method
The Human HS IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
- Homogenous (no wash): No
- Interassay CV: 6.7%
- Intraassay CV: 8.1%
Contents
- Pre-coated 96 well plate
- Standard
- Sample Diluent
- Assay Buffer concentrate
- Biotinylated Detection Antibody
- Amplification Diluent Concentrate
- Amplification Reagent I
- Amplification Reagent II
- SAV-HRP
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers
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