Invitrogen IL-10 Porcine ELISA Kit, 96 Tests

Invitrogen IL-10 Porcine ELISA Kit, 96 Tests

The Porcine Interleukin 10 (Por IL-10) ELISA quantitates Por IL-10 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Por IL-10.
Principle of the method
The Porcine IL10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV:  7.2%
• Intraassay CV: 5.1%

Contents    

• Sw IL-10 Standard, lyophilized, recombinant Sw IL‑10; contains 0.1% sodium azide. Refer to vial label for reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Plate, 96-well plate, 1 plate
• Sw IL‑10 Biotin Conjugate (Biotin‑labeled anti‑IL-10); contains 0.1% sodium azide, 11 mL
• Streptavidin‑HRP (100X), 0.15 mL
• Streptavidin‑HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3