Invitrogen IL-10RA (CD210a) Human ELISA Kit, 96 Tests

Invitrogen IL-10RA (CD210a) Human ELISA Kit, 96 Tests

The Human Interleukin 10 Receptor Alpha (CD210a) (Hu IL-10RA) ELISA quantitates Hu IL-10RA in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-10RA.
Principle of the method
The Human IL-10RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Human IL-10Ra Antibody Coated wells, 96-well plate, 1 plate
• Human IL-10Ra Biotin Conjugate, 2 vials
• Human IL-10Ra Standard, recombinant human IL-10Ra, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent C, 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (500X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2