Invitrogen IL-12 p40 Human ELISA Kit, 96 Tests

Invitrogen IL-12 p40 Human ELISA Kit, 96 Tests

The Human Interleukin-12 p40(Hu IL-12 p40) ELISA quantitates Hu IL-12 p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-12 p40.
Principle of the method
The Human IL-12 p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: 6.7%
• Intraassay CV: 4%

Contents    

• Anti-IL-12 + p40 Antibody-Coated Wells, 96-well strip-well plate, 1 plate
• Diluent Spec. (bovine plasma); for cell culture or urine, 2 vials
• Incubation Buffer; for serum/plasma, 11 mL
• Standard; 0 ng/mL in buffer with bovine serum, 1 vial
• Standards 1 to 5; in bovine serum; lyophilized. Refer to vial label for reconstitution volume and range, 5 vials
• Anti-IL-12-HRP Conjugate; in buffer with proteins, 6 mL
• Controls 1 and 2; in buffer with human plasma, 2 vials
• Wash Buffer Concentrate (200X), 10 mL
• Chromogenic TMB (tetramethylbenzidine) in DMF, 1 mL
• Substrate Buffer; H2O2 in acetate/citrate buffer, 3 vials
• Stop Solution (1 N HCl), 6 mL