Invitrogen IL-13 Human Matched Antibody Pair, 10 x 96 Tests

Invitrogen IL-13 Human Matched Antibody Pair, 10 x 96 Tests

The Human IL-13 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-13. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    

Contents    

• Capture Antibody: Pre-titrated, purified antibody
• Detection Antibody: Pre-titrated, biotin-conjugated antibody
• Standard: Recombinant cytokine for generating standard curve and calibrating samples
• ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
• Assay Diluent: 5X concentrated
• Detection enzyme: Pre-titrated Streptavidin-HRP
• Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
• Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
• 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -44, -76, -86)