Invitrogen IL-13 Porcine ELISA Kit, 5 x 96 Tests

Invitrogen IL-13 Porcine ELISA Kit, 5 x 96 Tests

The Porcine Interleukin 13 (IL-13) ELISA quantitates Porc IL-13 in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-13.
Principle of the method
The Porcine IL-13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Porcine IL-13 Antibody Coated wells, 96-well plate, 1 plate
• Porcine IL-13 Biotin Conjugate, 2 vials
• Porcine IL-13 Standard, recombinant porcine IL-13, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent (5X), 15 mL
• Streptavidin-HRP (800X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solutionl, 8 mL
• Adhesive Plate Covers, 2