Invitrogen IL-1RA Equine ELISA Kit, 96 Tests

Invitrogen IL-1RA Equine ELISA Kit, 96 Tests

The Equine Interleukin-1 Receptor Antagonist (Eq IL-1RA) ELISA quantitates Eq IL-1RA in equine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Eq IL-1RA.
Principle of the method
The Equine IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Equine IL-1RA Antibody Coated wells, 96-well plate, 1 plate
• Equine IL-1RA Biotin Conjugate, 2 vials
• Equine IL-1RA Standard, recombinant equine IL-1RA, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent C, 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (400X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2