Invitrogen IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor™ 660, 25 Tests, eBioscience™
On demandInvitrogen IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor™ 660, 25 Tests, eBioscience™
The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.
Staining for IL-21 has shown donor variability. stimulation times may vary depending on the donor and should be optimized. Increased staining levels have been observed on Th17-polarized normal human peripheral blood cells.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Features
- Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
- Recommended Isotype Control: Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™.
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