Invitrogen IL-4 Human ELISA Kit, HRP conjugated Antibody, 96 Tests

Invitrogen IL-4 Human ELISA Kit,  HRP conjugated Antibody, 96 Tests

The Human Interleukin 4 (Hu IL-4) ELISA quantitates Hu IL-4 in human serum. The assay will exclusively recognize both natural and recombinant Hu IL-4.
Principle of the method
The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Interassay CV: 3.8%
• Intraassay CV: 5.1%

Contents    

• Standard 0 in human serum, with preservatives (benzamidin and thymol). Lyophilized. Refer to vial label for quantity and reconstitute with 5 mL distilled water. 1 vial
• Standards 1 - 5 in human plasma, with preservatives (benzamidin and thymol).Lyophilized. Refer to vial label for concentration and reconstitute with 1 mL distilled water. Note: 1 pg of the standard preparation is equivalent to 10 mU NIBSC 88/656. 5 vials
• Controls 1 and 2 in human serum, with thymol. Lyophilized. Refer to vial label for range. Reconstitute with 1 mL distilled water. 2 vials
• Specimen Diluent (human serum with benzamidin and thymol). Lyophilized. Refer to vial label for reconstitution volume. 2 vials
• Incubation Buffer (Phosphate buffer with BSA and thymol). 11 mL per bottle. 1 bottle
• Anti-IL-4 Antibody-Coated Wells, 96 wells per plate. 1 plate
• Anti-IL-4-HRP Conjugate in buffered solution with bovine serum albumin and thymol; 6 mL per bottle. 1 bottle
• Wash Buffer Concentrate (200X); 10 mL per bottle. 1 bottle
• Concentrated Chromogen, Tetramethylbenzidine (TMB) in DMF, 1 mL per vial. 1 vial
• Substrate Buffer: H2 O 2 in acetate/citrate buffer; 21 mL per botttle. 3 bottles
• Stop Solution: H2 SO 4 , 1.8 N, 6 mL per bottle. 1 bottle