Invitrogen IL-6 Human ELISA Kit, 50 µL, 96 Tests, Sample With Urine

Invitrogen IL-6 Human ELISA Kit, 50 µL, 96 Tests, Sample With Urine

The Human Interleukin-6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6.
Principle of the method
The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <10%
• Intraassay CV: <10%

Contents    

• Anti-human IL-6 96-well Strip Plate, 1 each
• Recombinant Human IL-6 Standard, 2 vials
• Standard Diluent, contains 0.1% sodiumazide, 25mL
• Biotinylated Antibody Reagent, contains 0.1% sodiumazide, 8mL
• 30X Wash Buffer, 50mL
• Streptavidin-HRP Concentrate, 75μL
• Streptavidin-HRP Dilution Buffer, 14mL
• TMB Substrate, 13mL
• Stop Solution, contains 0.16M sulfuric acid, 13mL
• Adhesive plate covers, 6 each