Invitrogen IL-8 Human Matched Antibody Pair, 10 x 96 Tests

Invitrogen IL-8 Human Matched Antibody Pair, 10 x 96 Tests

The Human IL-8/NAP-1 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-8. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.

Principle of the method

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Storage: -20°C

Contents

• Capture (coating) Antibody
• Detection Antibody
• SAV-HRP conjugate
• Standard
• Sample Diluent