Invitrogen IL-8 Porcine ELISA Kit, 2 x 96 Tests, 100 µL

Invitrogen IL-8 Porcine ELISA Kit, 2 x 96 Tests, Sample Volume 100 µL

The Porcine Interleukin 8 (Por IL-8) ELISA quantitates Por IL-8 in porcine serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Por IL-8.

Principle of the method

The Porcine IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: 8.6%
• Intraassay CV: 7.1%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Swine IL‑8 Standard; lyophilized. Refer to vial label for quantity and reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Plate; 96-well strip-well plate, 1 plate
• Swine IL‑8 Biotin Conjugate; contains 0.1% sodium azide, 11 mL
• Streptavidin‑HRP (100X); contains 3.3 mM thymol, 0.125 mL
• Streptavidin‑HRP Diluent; contains 3.3 mM thymol, 25 mL
• Incubation Buffer, 12 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3