Invitrogen™ Immune Monitoring 65-Plex Human ProcartaPlex™ Panel, 384 well (lyophilized beads)

Invitrogen™ Immune Monitoring 65-Plex Human ProcartaPlex™ Panel, 384 well (lyophilized beads)

The Human Immune Monitoring 65-Plex ProcartaPlex Panel, 384 well (lyophilized beads) enables the study of immune function by analyzing 65 protein targets in a single well using Luminex FLEXMAP 3D and INTELLIFLEX instruments. Analyze 65 cytokine, chemokine, and growth factor targets simultaneously for efficient immune response profiling, biomarker discovery, and validation. The 384-well flat bottom plate contains ready-to-use lyophilized Luminex beads for fewer pipetting steps, less time-to-result, and seamless integration into workflows.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 6.3–12.5 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

About ProcartaPlex 384-well assays for the Luminex platform, ProcartaPlex 384-well immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using Luminex FLEXMAP 3D and INTELLIFLEX instruments. ProcartaPlex 384-well multiplex assays require as little as 6.3 µL of plasma or serum, or 12.5 µL of cell culture supernatant, and just four hours to obtain analyzed results.
The Luminex beads in the ProcartaPlex 384-well assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., FLEXMAP 3D and INTELLIFLEX). Similar to a sandwich ELISA, the ProcartaPlex 384-well assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the FLEXMAP 3D instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

Target list [bead region]:

• Cytokines: G-CSF (CSF-3) [42], GM-CSF [44], IFN alpha [48], IFN gamma [43], IL-1 alpha [62], IL-1 beta [18], IL-2 [19], IL-3 [73], IL-4 [20], IL-5 [21], IL-6 [25], IL-7 [26], IL-8 (CXCL8) [27], IL-9 [52], IL-10 [28], IL-12p70 [34], IL-13 [35], IL-15 [65], IL-16 [70], IL-17A (CTLA-8) [36], IL-18 [66], IL-20 [81], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14], IL-31 [37], LIF [15], M-CSF [67], MIF [53], TNF alpha [45], TNF beta [54], TSLP [80]
• Chemokines: BLC (CXCL13) [29], ENA-78 (CXCL5) [82], Eotaxin (CCL11) [33], Eotaxin-2 (CCL24) [37], Eotaxin-3 (CCL26) [77], Fractalkine (CX3CL1) [59], Gro-alpha (CXCL1) [61], IP-10 (CXCL10) [22], I-TAC (CXCL11) [57], MCP-1 (CCL2) [51], MCP-2 (CCL8) [8], MCP-3 (CCL7) [68], MDC (CCL22) [87], MIG (CXCL9) [69], MIP-1 alpha (CCL3) [12], MIP-1 beta (CCL4) [47], MIP-3 alpha (CCL20) [56], SDF-1 alpha (CXCL12) [13]
• Growth factors/regulators: FGF-2 [75], HGF [46], MMP-1 [64], NGF beta [55], SCF [39], VEGF-A [78]
• Soluble receptors: APRIL [88], BAFF [86], CD30 [84], CD40L (CD154) [74], IL-2R (CD25) [22], TNF-RII [85], TRAIL (CD253) [58], TWEAK [97]

Features

• 384-well flat bottom plates with ready-to-use lyophilized Luminex beads for fewer pipetting steps, less time-to-result, and seamless integration into workflows
• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 6.3–12.5 µL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

Contents

• Human Standard Mix M (lyophilized)
• 384-well plate with lyophilized Capture Bead Mix (1X)
• Biotinylated Detection Antibody Mix (1X)
•  Human Control-High M
• Human Control-Low M
• Reading Buffer (1X)
• Wash Buffer (10X)
• Streptavidin-PE (1X)
• Universal Assay Buffer (1X)
• 8-tube strip
• Adhesive film