Invitrogen Interferon alpha (Cell culture) Human ELISA Kit, 5 x 96 Tests

Invitrogen Interferon alpha (Cell culture) Human ELISA Kit, 5 x 96 Tests

The Human Interferon alpha A, 2, A/D, D, K and 4b (Hu IFNαA, IFNα2, IFNαA/D, IFNαD, IFNαK and IFNα4b ) ELISA quantitates Hu IFNα in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFNα.

Principle of the method

The Human IFNα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <8%
• Intraassay CV: <8%
• Storage: 2-8°C

Contents    

• Pre-coated 96 well Microplate
• Detection Antibody Concentrate
• Anti-IgG-HRP Concentrate
• Concentrate Diluent
• Standard
• Wash Buffer Concentrate
• Substrate Solution
• Sample Diluent
• Stop Solution
• Adhesive Plate Covers