Invitrogen IR (Phospho) [pY1158] Human ELISA Kit

Invitrogen IR (Phospho) [pY1158] Human ELISA Kit

The Human IR (Phospho) [pY1158] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of IR (Phospho) [pY1158] in fresh or frozen human cell lysates. Cross-reactivity with mouse and rat cells has been observed. The assay recognizes both natural and recombinant human IR (Phospho) [pY1158].

Principle of the method

A monoclonal capture antibody specific for IR (Phospho) [pY1158] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.

Features

• Homogenous (no wash): No
• Interassay CV: 7.5%
• Intraassay CV: 5.9%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Pre-coated 96 well plate
• Standard
• Standard Dilution Buffer
• Detection Antibody
• anti-rabbit-HRP
• HRP Diluent
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers