Invitrogen JAK2 (Phospho) [pY1007/pY1008] Human ELISA Kit, 96 Tests

Invitrogen JAK2 (Phospho) [pY1007/pY1008] Human ELISA Kit, 96 Tests

The Human JAK2 (Phospho) [pY1007/pY1008] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of JAK2 (Phospho) [pY1007/pY1008] in fresh or frozen human or mouse cell lysates. The assay recognizes both natural and recombinant JAK2 (Phospho) [pY1007/pY1008].
Principle of the method
A monoclonal capture antibody specific for JAK2 (Phospho) [pY1007/pY1008]) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.

• Homogenous (no wash): No    
• Interassay CV: 7.5%
• Intraassay CV: 4.5%

Contents    

• Pre-coated 96 well plate
• Standard
• Standard Dilution Buffer
• Detection Antibody
• anti-rabbit-HRP
• HRP Diluent
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers