Invitrogen LIX-Cell Lysate (CXCL5) Rat ELISA Kit, 10 x 96 Tests

Invitrogen LIX-Cell Lysate (CXCL5) Rat ELISA Kit, 10 x 96 Tests

The Rat LIX (CXCL5) ELISA quantitates Rt LIX in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt LIX.

Principle of the method

The Rat LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: <12%
• Intraassay CV: <10%
• Homogenous (no wash): No
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Rat LIX lysates Antibody Coated wells, 96-well plate, 1 plate
• Rat LIX lysates Biotin Conjugate, 2 vials
• Rat LIX lysates Standard, recombinant rat LIX lysates, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Sample Diluent Buffer (5X), 10 mL
• Assay Diluent (5X), 15 mL
• Cell lysate buffer (2X), 5 mL
• Streptavidin-HRP (400X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2